ISOLATION AND CHARACTERIZATION OF MICROORGANISMS FROM STORED PAP (OGI)

ISOLATION AND CHARACTERIZATION OF MICROORGANISMS FROM STORED PAP (OGI)   

 ABSTRACT:   

The microbial changes which took place during the steeping and storage of pan (ogi) was studied.  The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping.  The bacterial  number for the steeped water ranged from 4.7x104  to 3.2 x 107 cfu/ml while the fungal number ranged from 1.5 x103 to 5.7 x 106 cfu/ml.  In the stored pap, pap I had higher count which range from 7.2x104 to 6.0x107 cfu/g while 6.0x107 to 1.6x1011. The fungal count for pap I and II ranged from 8.2 x 105 to 2.5x1012 and 8.2x106 to 3.6x1010 respectively. The bacteria isolated from stee pingwere Lactabacillus species Bacillus specie,  Corynebacterium,  streptococcus specie and clostridium species.  The fungi were Aspergillus species fusrarium specie, pencillium specie sachanomyces specie and candida specie.  The bacteria spp isolated from stored pap were lactobacillus species, streptococcus specie  Eubacterium specie,  Pseudomonas specie, Baccillus specie, streptococcus specie, Lactobacillus specie, Leucomostoc specie for pap I and II respectively.  The fungi isolated were Aspergillus specie, Pensillum specie, fusarium specie, sacharomyces specie, candida specie, Debaryomyces specie for pap I and II respectively. The pap stored without changing water developed an off flavour after 48 hours and is not fit for consumption.  On the other hand, the pap stored while changing water is fit and better for consumption. 

LIST OF TABLES

Table I:The percentage chemical composition of maize grain9

Table 2: changes in PH value during the steeping of maize for pop production 24

Table 3: Bacterial count of steeped water during the steeping of maize for pap production 25

Table 4: Fungal counts of steeped water during the steeping of maize for pap production 26

Table 5: Bacterial count for pap samples I and II during storage at room temperature 26

Table 6:Fungal count for pap samples I and II during storage at room temperature27

Table 7: Predominant bacteria isolated from steeped water   29

Table 8: Fungi isolated from steeped water 30

Table 9: Predominant bacteria isolated from stored pap I       34

Table 10: Predominant bacteria isolated from pap II 35

Table 11: Fungal isolated from samples Pap I 36

Table 12: Fungal isolated from sample Pap II 36

LIST OF FIGURES

Figure 1: Structure of maize grain------------------------------

Figure 2: Steps in preparation of pap (Oge)-------------------

Figure 3: Changes in PH value during the steeping of maize 

for pap production

TABLE OF CONTENTS

Title page

Certification

Dedication

Acknowledgement

Abstract

List of tables

List of figures

Table of contents

CHAPTER ONE:   INTRODUCTION

1.1 Background of the study

1.2 Statement of problem

1.3 Aim and objectives of the study

1.4 Hypothesis

1.5 Justification of the study

1.6 Significance of study

1.7 Limitation of the study

CHAPTER TWO:   LITERATURE

2.1 Origin of maize pad

2.2 Structure of maize grain

2.3 Chemical composition of maize grain

2.4 Uses of maize pap

2.5 Chemical changes in stored pap (ogi)

2.6 Nutrition changes in stored pap

2.7 Microorganisms associated with stored pap

CHAPTER THREE:  MATERIALS AND METHOD

3.1 Collection of sample

3.2 Materials and equipments used

3.3 Media and reagents used

3.4 Preparation of pap (ogi)

3.5 Quantitative analysis of microorganisms

3.5.1 Serial dilution

3.5.2 Culturing technique

3.6 Isolation of bactria

3.7 Characterization of isolate

3.8 Biochemical test for identification of microorganisms

3.9 Identification of fungi

CHAPTER FOUR:   RESULT AND DISCUSSION

CHAPTER FIVE:  

5.1 Conclusion

5.2 Recommendation

References

Appendix

CHAPTER ONE

INTRODUCTION

1.1 BACKGROUND OF THE STUDY

Cereals have been known to man from the earliest times porridge prepared from cereals are  eaten in different parts o the world, especially in developing countries where they may present the basic diet.  This porridge could be baked to enhance the taste, quality and improve digestibility (Oke 1967, Adeniyi and Potter 1978, Uno and field 1981).

Corn (zea mays) is one of cereals which is an important raw material in human diet.  In Nigeria, maize is grown mainly in the southern part of Nigeria while sorghum (Sorghum bicolor) and millet (perinisetum typhoideum) are grown in the northern part of the country. A fermented cereal product is known as paplogi).  Corn is processed into traditional food such as paplogi) Umo and fields 1981).

Pap is a fermented non-alcoholic starchy food and is a major staple food widely consumed in west Africa.  It is a sour fine past beverage which when cooked produces a thin semi solid porridge. Pap (Ogi) porridge has a smooth texture and a sour taste resembling that of yoghurt,.  In Nigeria, some states such as Anambra, Imo, Enugu and Abia refer to it as Akamu but Ogi is a Yoruba name but most state of Nigeria, it refers to maize pap.  On the other hand, sorghum pap is known as Ogi baba while millet pap is known as Ogi gero in Yoruba (Banigi 1977, Onyekwere and Akinrele 1977).

Pap logi) can be consumed with variety of other product including with bread, steamed been cake (moi-moi), fried bean cake (Akara), fried yam and plantain etc.  it is used as a main meal for adult and sick patients and it is suitable for breakfast, lunch and dinner.  Pap is widely used as the first native food given to babies at wearing to supplement breast milk and is a major breakfast cereal for pre-school children and adults.   It is consumed as a main meal for convalescing patients because it can easily be digested.  As a wearing food, it is utilized mainly by low income earners category, it is estimated that about 25 million or more adults eat it about 4-5 days weakly (Banigo 1972).  Milk and sugar may be added to improved the taske and nutritional quality.  Pap is cooked and turned into a stiff gel called Agidi which is similar to kenkey, a fermented shanian product (Muller 1988, withby 1968).  Some Yoruba indigens beliwved that pap is capable of stimulating the production of breast milk in Nursing mother (Bassir 1962).  However, there  has been no qualitative evidence of support of this belief.

In spite of it’s important in the Nigeria diet, pap manufacture is essential a home based industry.  There are at present no large scale factory operation for the production of pap.  The manufacture is carried out on a small scale by some house wives as a commercial venture in many parts of the country.  The cleaned grain free of dirt and impurities steeped in eastern ware, plastic or enamel pot for 1-3 days at room temperature, this is followed by wet milling and sieving.  Twenty-four (24) hours of sleeping leads to a greater depletion of the fermentable carbohydrate.  After sieving, the coarse material obtained is wash with water to separate more of the starch.  The filtered slurry is allowed to sediment and undergo further fermentation for 1-2 days at room temperature.  The coarse matter which is separated is used ad animal feed while the sediment (Pap) is boiled to obtain.

1.2 STATEMENT OF PROBLEM

Microorganisms are involved in the processing of pap especially during fermentation and equally during storage.  Few organisms are found in the pap, if it is properly stored.  Their presence in pap during storage leads to irregular of flavour and loss of nutrients (van veen and steinkrans 1990).  This is the result of their metabolic activity in the stored pap.

The tradiitonal method of pap production using various grains encourage significant nutrient losses, Losses m,ay occur during steeping, milling and sieving.  Large parts of the protein in the grain is located in the testa and germ that are shifted off during processing.

Losses of fibre, protein, ash as well as some vitamins have been reported by Banigo and Muller (1972). Losses in nutrients could be minimized by using an improved wet milling method devised by Banigo and Muller (1972).  At the end of this work, the microorganisms found in stored pap was isolated and characterized.

1.3 AIM AND OBJECTIVES OF THE STUDY

The aim of this study is to isolate and characterize micro-organisms from stored pap.

SPECIFIC OBJECTIVES ARE

i. To isolate and characterize bacterial contaminants from stored pap

ii. To isolate and characterize fungal contaminants from stored pap

1.4 HYPOTHESIS

HI:Bacteria and fungi are involved in the fermentation and storage of maize pap.

1.5 JUSTIFICATION OF THE STUDY

Not very much work has been done in this in recent times, with reference to the microbiology of the process.  Some of the earliest workdone in this include those of Akiurele (1970-1977) and Barigo (1969, 1970, 1972, 1977).

1.6 SIGNIFICANCE OF STUDY

Pap stored improperly for a relatively length of time is likely to develop microorganisms.  Therefore, the study is carried-out to identify this organisms and properly advice on the proper way of storing pap.

1.7 LIMITATION OF THE STUDY

This work is limited to viable microorganism present in the corn (zea mays) only which will be purchased from Ogbete in the Enugu Area.